KMID : 0379120040320010016
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Korean Journal of Mycology 2004 Volume.32 No. 1 p.16 ~ p.22
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Isolation of Gibberellin-producing Penicillium spp. from the Root of Lindera obtusiloba and Vaccinium koreanum
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Choi Hwa-Yeol
Lee Jin-Hyung Lee Jin-Hyung Kim Jong-Guk Shin Kee-Sun Lee In-Gu
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Abstract
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The gibberellins (GAs) play important roles in plant growth and development. Twenty three fungi were isolated from the roots of Lindera obtusiloba and Vaccinium koreanum. The numbers of GA-producing fungi were six strains from Lindera obtusiloba and four strains from Vaccinium koreanum. The fungi with GAs-producing activity were incubated for seven days in 40 ml of Czapek¡¯s liquid medium at $25^{\circ}C$, 120 rpm, and the amount of each GA in the medium was measured by gas chromatography-mass spectrometer (GC-MS) to determine the productivity of GAs. Penicillium griseofulvum KNU5379 produced more GA in case of $GA_{3}$ than Neurospora crassa known as a GAs-producing fungus. P. griseofulvum KNU5379 was shown to produce $GA_1\;9.79\;ng,\;GA_3\;133.58\;ng,\;GA_4\;2.64\;ng,\;GA_7\;7.80\;ng\;and\;GA_{53}\;0.73\;ng$ in 25 ml of liquid medium. Bioassay using culture fluid of GAs-production fungi was performed on rice sprout.
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KEYWORD
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Bioassay, Gibberellins
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